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Improvement of biohydrogen production by Enterobacter cloacae IIT-BT 08 under regulated pH

Paper ID Volume ID Publish Year Pages File Format Full-Text
24098 43497 2011 7 PDF Available
Title
Improvement of biohydrogen production by Enterobacter cloacae IIT-BT 08 under regulated pH
Abstract

The present study investigates the effect of pH and intermediate products formation on biological hydrogen production using Enterobacter cloacae IIT-BT 08. Initial pH was found to have a profound effect on hydrogen production potential, while regulating the pH 6.5 throughout the fermentation was found to increase the cumulative hydrogen production rate and yield significantly. Modified Gompertz equation was used to fit the cumulative hydrogen production curves to obtain the hydrogen production potential P, the hydrogen production rate R and lag phase λ. At regulated pH 6.5, higher H2 yield (3.1 mol H2 mol−1 glucose), specific hydrogen production potential (798.1 mL/g) and specific rate of H2 production (72.1 mL L−1 h−1 g−1) were obtained. The volatile fatty acid profile showed butyrate, ethanol and acetate as the major end metabolites of fermentation under the operating pH conditions tested; however, their pattern of distribution was pH dependent. At the optimum pH of 6.5, the acetate to butyrate ratio (A/B ratio) was found to be higher than that at any other pH. The study also investigates the effect of sodium ions on biohydrogen production potential. It was also found that sodium ion concentration up to 250 mM enhanced the hydrogen production potential; however, any further increase in the metal ion concentration had an inhibitory effect.

Keywords
Biohydrogen production; Enterobacter cloacae IIT-BT 08; pH; Sodium ions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 152, Issues 1–2, 10 March 2011, Pages 9–15
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us