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Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration

Paper ID Volume ID Publish Year Pages File Format Full-Text
241 20 2015 9 PDF Available
Title
Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration
Abstract

Several diseases can lead to opacification of cornea requiring transplantation of donor tissue to restore vision. In this context, transparent collagen I fibrillated matrices have been synthesized at 15, 30, 60 and 90 mg/mL. The matrices were evaluated for fibril organizations, transparency, mechanical properties and ability to support corneal epithelial cell culture. The best results were obtained with 90 mg/mL scaffolds. At this concentration, the fibril organization presented some similarities to that found in corneal stroma. Matrices had a mean Young’s modulus of 570 kPa and acellular scaffolds had a transparency of 87% in the 380–780 nm wavelength range. Human corneal epithelial cells successfully colonized the surface of the scaffolds and generated an epithelium with characteristics of corneal epithelial cells (i.e. expression of cytokeratin 3 and presence of desmosomes) and maintenance of stemness during culture (i.e. expression of ΔNp63α and formation of holoclones in colony formation assay). Presence of cultured epithelium on the matrices was associated with increased transparency (89%).

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Keywords
Collagen; Liquid-crystal; Transparent matrices; Human corneal epithelium; Limbal stem cell
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Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 22, August 2015, Pages 50–58
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us