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Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene–water biphasic system

Paper ID Volume ID Publish Year Pages File Format Full-Text
24100 43497 2011 6 PDF Available
Title
Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene–water biphasic system
Abstract

The enantioselective hydrolysis of mandelonitrile with whole cells of a recombinant Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high concentrations (>300 mM), which resulted in a low yield of the target product (R)-(−)-mandelic acid. To relieve the substrate inhibition and to enhance the (R)-(−)-mandelic acid productivity, eight water–organic solvent biphasic systems were attempted in this work. Toluene was found to be the most suitable solvent as the organic phase among the solvents tested. Various parameters were systematically examined and optimized in shake flasks. The phase volume ratio, buffer pH and reaction temperature were shown to be sensitive parameters affecting both the yield and the enantiopurity of product in the biphasic system. Under the optimized conditions, significant enhancement of substrate tolerance from 200 mM to 500 mM and average productivity from 179.6 g l−1 d−1 to 352.6 g l−1 d−1 were achieved. Subsequently, the biocatalytic hydrolysis of mandelonitrile was successfully carried out in a stirred reactor (2-l scale) by repeated use of the calcium alginate entrapped cells for 5 batches, affording 110.7 g (R)-(−)-mandelic acid in 98.0% ee (enantiomeric excess) and a specific production of 13.8 g (mandelic acid) g−1 (cell), respectively.

Keywords
Aqueous-organic biphasic system; Recombinant nitrilase; Enantioselective hydrolysis; High substrate tolerance; (R)-(−)-Mandelic acid; Escherichia coli whole cells
First Page Preview
Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene–water biphasic system
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 152, Issues 1–2, 10 March 2011, Pages 24–29
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering