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A novel vector for lactic acid bacteria that uses a bile salt hydrolase gene as a potential food-grade selection marker

Paper ID Volume ID Publish Year Pages File Format Full-Text
24104 43497 2011 5 PDF Available
Title
A novel vector for lactic acid bacteria that uses a bile salt hydrolase gene as a potential food-grade selection marker
Abstract

A novel vector pM4aB for lactic acid bacterial was developed using a bile salt hydrolase gene from Lactobacillus plantarum as a potential food-grade selection marker. The 3.0-kb pM4aB consisted of the replicon of Lactobacillus plasmid pM4, a multiple cloning site and the bsh gene, which was constructed by elimination of a 5.5-kb non-food-grade DNA fragment from an 8.5-kb intermediate vector pBEmpM4aB. For electroporation into Lactobacillus paracasei X9, a high transformation efficiency of 4.0 ± 1.0 × 104 CFU/μg plasmid DNA was yielded with 0.1% (wt/vol) glycodeoxycholic acid sodium selection. A high segregation stability of the vector was also observed as only 0.1% plasmid was lost after 50 generations of growth without selection pressure. The application potential of pM4aB was further confirmed by expression of a catalase gene from Lactobacillus sakei in L. paracasei. These results revealed that the novel vector pM4aB constructed in this study would be a useful tool for genetic modification of the industrially important LAB.

Keywords
Bile salt hydrolase; Food-grade vector; Selection marker; Lactic acid bacteria
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A novel vector for lactic acid bacteria that uses a bile salt hydrolase gene as a potential food-grade selection marker
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 152, Issues 1–2, 10 March 2011, Pages 49–53
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us