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Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media

Paper ID Volume ID Publish Year Pages File Format Full-Text
24127 43499 2011 7 PDF Available
Title
Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media
Abstract

A liquid chromatography–mass spectrometry (LC–MS) based metabolomics platform was previously established to identify and profile extracellular metabolites in culture media of mammalian cells. This presented an opportunity to isolate novel apoptosis-inducing metabolites accumulating in the media of antibody-producing Chinese hamster ovary (CHO mAb) fed-batch bioreactor cultures. Media from triplicate cultures were collected daily for the metabolomics analysis. Concurrently, cell pellets were obtained for determination of intracellular caspase activity. Metabolite profiles from the LC–MS data were subsequently examined for their degree of correlation with the caspase activity. A panel of extracellular metabolites, the majority of which were nucleotides/nucleosides and amino acid derivatives, exhibited good (R2 > 0.8) and reproducible correlation. Some of these metabolites, such as oxidized glutathione, AMP and GMP, were later shown to induce apoptosis when introduced to fresh CHO mAb cultures. Finally, metabolic engineering targets were proposed to potentially counter the harmful effects of these metabolites.

Keywords
Metabolomics; Chinese hamster ovary; Liquid chromatography–mass spectrometry; Apoptosis; Metabolic engineering
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Metabolomics-based identification of apoptosis-inducing metabolites in recombinant fed-batch CHO culture media
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 151, Issue 2, 20 January 2011, Pages 218–224
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Full-text PDF Download
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