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Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase

Paper ID Volume ID Publish Year Pages File Format Full-Text
24141 43500 2010 6 PDF Available
Title
Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase
Abstract

This paper reports on the cross-linking and immobilisation of various proteins by the recombinant tyrosinase from Verrucomicrobium spinosum (Vs-tyrosinase). In general it is found that Vs-tyrosinase can readily cross-link proteins with a low degree of complexity, such as casein, but that the enzyme cannot readily cross-link well folded protein substrates such as lysozyme, myoglobin, cytochrome c or Candida antarctica lipase B (CALB). However, the inclusion of phenolic compounds (phenol or caffeic acid) to reaction mixtures of these proteins can greatly enhance the levels of cross-linking. For example it is possible to prepare cross-linked aggregates of industrially applicable enzymes such as CALB by simply incubating it with Vs-tyrosinase and phenol. The resulting aggregates can be collected by centrifugation and retain high levels of activity and may find applications in biocatalysis.

Keywords
Tyrosinase; Verrucomicrobium spinosum; Protein cross-linking; Immobilised enzymes; Lipase
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Cross-linking and immobilisation of different proteins with recombinant Verrucomicrobium spinosum tyrosinase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 4, December 2010, Pages 546–551
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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