fulltext.study @t Gmail

Deactivation and unfolding are uncoupled in a bacterial lipase exposed to heat, low pH and organic solvents

Paper ID Volume ID Publish Year Pages File Format Full-Text
24148 43501 2009 5 PDF Available
Title
Deactivation and unfolding are uncoupled in a bacterial lipase exposed to heat, low pH and organic solvents
Abstract

The lipase from Burkholderia glumae (BGL) was incubated at variable temperature, pH and concentration of organic solvents, and the decrease of enzymatic activity was compared to changes in the molecular structure as monitored by ESI-mass spectrometry. We observed that deactivation is not strictly related to structural instability in the assay conditions, in fact (i) thermal deactivation preceded denaturation; (ii) acid-induced deactivation arose at higher pH than partial or global protein unfolding; and (iii) activity in most organic solvents decreased at solvent concentrations where conformation was fully retained. In particular, no denaturation at all could be elicited by dimethyl formamide (DMF), isopropanol, and dimethyl sulfoxide (DMSO) up to 80%, in spite of a reduction of enzyme activity to 60–75%.

Keywords
Burkholderia glumae lipase; ESI-mass spectrometry; Stability; Aggregation; Inactivation
First Page Preview
Deactivation and unfolding are uncoupled in a bacterial lipase exposed to heat, low pH and organic solvents
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 141, Issues 1–2, 20 April 2009, Pages 42–46
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us