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A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
24157 43502 2010 6 PDF Available
Title
A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli
Abstract

Unique properties of bacteriorhodopsin, namely, photochromism and high thermal stability, make this protein an attractive target for physico-chemical studies, as well as for various biotechnological applications. Using Mistic as a suitable carrier for insertion of recombinant membrane proteins into cytoplasmic membrane of Escherichia coli, we developed a system for overexpression of bacteriorhodopsin and worked out an efficient procedure for its purification and renaturation with the final yield of 120 mg/l of refolded protein, which is the highest value reported to date for bacteriorhodopsin produced in E. coli. Functional activity of recombinant bacteriorhodopsin was confirmed by spectroscopic and electrochemical assays.

Keywords
bR, bacteriorhodopsin; bO, bacterio-opsin; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-l-propanesulfonate; DMPC, l-a-dimyristoyl-sn-glycerophosphatidylcholine; NG, n-nonyl-β-d-glucopyranoside; Sarkosyl, sodium N-lauroylsarcosine; IPTG, isopropyl-β-d-thi
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A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 147, Issues 3–4, June 2010, Pages 145–150
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us