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Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

Paper ID Volume ID Publish Year Pages File Format Full-Text
24171 43503 2010 5 PDF Available
Title
Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis
Abstract

Bacillus thuringiensis produces crystal proteins (Cry) that account for up to 25% of the dry cell weight during the stationary phase. The high-level expression and stationary phase-specific autoinduction of the cry gene led to development of a cry promoter-based Bacillus expression system. Among the various cry promoters, cry3Aa promoter was selected by comparing the lacZ expression levels in Bacillus subtilis. An extracellular enzyme cellulase was highly upregulated during the stationary phase while under control of the cry3Aa promoter. Improvement of the cry3Aa promoter was obtained by modification of the promoter sequence. Specifically, a 5-fold increase in lacZ expression was obtained by changing both the −35 and −10 boxes of the cry3Aa promoter to the consensus sequence of the σA-dependent promoter of B. subtilis. The modified cry3Aa promoter produced a significantly higher yield of AprE, which suggests that the promoter may be useful for high-level protein expression in B. subtilis.

Keywords
Bacillus thuringiensis cry3Aa; Expression system; Promoter modification; Bacillus subtilis
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Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issues 1–2, 20 August 2010, Pages 16–20
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us