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A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms

Paper ID Volume ID Publish Year Pages File Format Full-Text
24172 43503 2010 3 PDF Available
Title
A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms
Abstract

Recombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins.

Keywords
Cloning strategy; Expression vectors; Native protein; Recombinant protein; Fusion tag; Biocatalysts
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A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issues 1–2, 20 August 2010, Pages 21–23
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us