fulltext.study @t Gmail

Optimizing the secondary structure of human papillomavirus type 16 L1 mRNA enhances L1 protein expression in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
24186 43504 2010 6 PDF Available
Title
Optimizing the secondary structure of human papillomavirus type 16 L1 mRNA enhances L1 protein expression in Saccharomyces cerevisiae
Abstract

Human papillomavirus (HPV) type 16 is the most significant type in the prophylactic vaccine field because 50–60% of cervical cancer cases are caused by infection with HPV type 16. The L1 protein, which is the highly immunogenic major capsid protein of HPV, is the major component of the prophylactic vaccine. To enhance L1 protein expression in Saccharomyces cerevisiae we used a strategy involving the modification of the HPV16 L1 gene (MO) to reduce the amount of predicted mRNA secondary structure, rather than a strategy aiming at optimizing codon bias as has often been used to enhance the expression of foreign genes. The mean expression level of L1 protein in MO-transformed candidates was four-fold higher than in cells transformed with the wild type HPV16 L1 gene (WT), while the fraction of L1 mRNA transcribed from MO in total RNA was four-fold lower than with L1 mRNA transcribed from WT. This demonstrates that L1 protein expression in MO-transformed cells was increased by a post-transcriptional mechanism. The L1 protein produced in MO-transformed cells self-assembled into virus-like particles (VLP) with features similar to those of the native virion. These results indicate that the strategy of optimizing the secondary structure of HPV16 L1 mRNA was successful in increasing L1 protein expression in Saccharomyces cerevisiae.

Keywords
Human papillomavirus; Cervical cancer; mRNA secondary structure; Virus-like particles; Saccharomyces cerevisiae
First Page Preview
Optimizing the secondary structure of human papillomavirus type 16 L1 mRNA enhances L1 protein expression in Saccharomyces cerevisiae
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 1, 1 October 2010, Pages 31–36
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us