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Simultaneous detection of Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma genitalium and Mycoplasma hominis by fluorescence polarization

Paper ID Volume ID Publish Year Pages File Format Full-Text
24188 43504 2010 3 PDF Available
Title
Simultaneous detection of Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma genitalium and Mycoplasma hominis by fluorescence polarization
Abstract

A simple and practical method for simultaneous detection of Ureaplasma urealyticum (U. urealyticum), Ureaplasma parvum (U. parvum), Mycoplasma hominis (M. hominis) and Mycoplasma genitalium (M. genitalium) by fluorescence polarization (FP) assay has been developed. DNA of the 16S rRNA gene of U. urealyticum, U. parvum, M. genitalium and M. hominis was amplified using a forward primer and two reverse primers in an asymmetric PCR. Four probes labeled with different fluorophores were specific for U. urealyticum, U. parvum, M. hominis and M. genitalium, respectively. The probes hybridized with their target PCR products, and the hybridization increased the fluorescence polarization (FP) values. The pathogens were identified by the increased FP values. DNA extracted from 757 urethral samples was detected by the FP assay and the sequence method. The FP assay could detect co-infection more effectively than the sequence method did. The method presented in this study was expected to be extremely useful in clinic.

Keywords
Fluorescence polarization; Mycoplasma genitalium; Mycoplasma hominis; Ureaplasma urealyticum; Ureaplasma parvum
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Simultaneous detection of Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma genitalium and Mycoplasma hominis by fluorescence polarization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 1, 1 October 2010, Pages 41–43
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us