fulltext.study @t Gmail

Limiting factors of the translation machinery

Paper ID Volume ID Publish Year Pages File Format Full-Text
24189 43504 2010 7 PDF Available
Title
Limiting factors of the translation machinery
Abstract

Cell-free production of proteins is a rapid developing technology for many applications in proteomic sciences. Although great progress was made in the last years, in vitro protein synthesis is still less efficient than in vivo protein synthesis. For Escherichia coli based systems, all factors needed for cell-free protein synthesis are established, as shown in the reconstituted PURE system. Here we report the influence of additional translation factors, aminoacyl-tRNA synthetases and translational active ribosomes on cell-free protein synthesis of E. coli S30 extracts, indicating that none of these factors is a limiting factor for the protein synthesis. Furthermore, we show that varying the ratio of ribosomes including associated factors to other factors present in the extracts could not increase the yield of protein synthesized. In summary our results provide strong evidence for an optimal reconstitution of the translation machinery in E. coli S30 extracts.

Keywords
Cell-free protein synthesis; Translation factors; Escherichia coli; S30 extract
First Page Preview
Limiting factors of the translation machinery
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 150, Issue 1, 1 October 2010, Pages 44–50
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us