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An information theoretic thresholding method for detecting protein colocalizations in stacks of fluorescence images

Paper ID Volume ID Publish Year Pages File Format Full-Text
24217 43505 2010 5 PDF Available
Title
An information theoretic thresholding method for detecting protein colocalizations in stacks of fluorescence images
Abstract

In this note, we present a new method that allows us to determine threshold values for separating presence and absence of proteins in a stack of fluorescence images describing a spatial distribution of proteins across a biological object (like a slice of nervous tissue, a sample of blood cells, etc.). We apply this method to stacks of fluorescence images and find that the resulting threshold values are almost identical with threshold values found using completely independent methods based on technological and biological aspects of the images in question.

Keywords
MELK technology; Kullback–Leibler distance; Threshold values; Numerical optimization
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An information theoretic thresholding method for detecting protein colocalizations in stacks of fluorescence images
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 149, Issue 3, 1 September 2010, Pages 127–131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us