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Identification and expression analysis of miRNAs during batch culture of HEK-293 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24240 43507 2009 7 PDF Available
Title
Identification and expression analysis of miRNAs during batch culture of HEK-293 cells
Abstract

MicroRNAs (miRNAs) are small non-coding RNAs of about 20–24 nucleotides in length. They regulate gene expression negatively and have been implicated in a wide variety of biological processes. To identify potential miRNAs that may influence the growth and proliferation of mammalian cells cultured in bioreactors, we applied miRNA microarray expression profiling technology to batch cultures of HEK293 cells in protein free media. In our study, we identified miRNAs that were differentially expressed during the exponential and stationary phases, 13 of these showed distinct up regulation trends while 1 exhibited down regulation. These miRNAs have been implicated in cellular differentiation, growth arrest and apoptosis. Specifically, miR-16 and let-7b are potentially useful in the enhancement of bioreactor cell cultures.

Keywords
microRNA (miRNA); HEK293; Batch cell culture; CHO
First Page Preview
Identification and expression analysis of miRNAs during batch culture of HEK-293 cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 140, Issues 3–4, 25 March 2009, Pages 149–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering