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Microporous bacterial cellulose as a potential scaffold for bone regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
2430 112 2010 8 PDF Available
Title
Microporous bacterial cellulose as a potential scaffold for bone regeneration
Abstract

Nanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300–500 μm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young’s modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.

Keywords
Bacterial cellulose; Porogen; MC3T3-E1; Bone tissue engineering; Mineralization
First Page Preview
Microporous bacterial cellulose as a potential scaffold for bone regeneration
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 7, July 2010, Pages 2540–2547
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering