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Design and prototyping of a chip-based multi-micro-organoid culture system for substance testing, predictive to human (substance) exposure

Paper ID Volume ID Publish Year Pages File Format Full-Text
24367 43509 2010 6 PDF Available
Title
Design and prototyping of a chip-based multi-micro-organoid culture system for substance testing, predictive to human (substance) exposure
Abstract

Dynamic miniaturized human multi-micro-organ bioreactor systems are envisaged as a possible solution for the embarrassing gap of predictive substance testing prior to human exposure. A rational approach was applied to simulate and design dynamic long-term cultures of the smallest possible functional human organ units, human “micro-organoids”, on a chip the shape of a microscope slide. Each chip contains six identical dynamic micro-bioreactors with three different micro-organoid culture segments each, a feed supply and waste reservoirs. A liver, a brain cortex and a bone marrow micro-organoid segment were designed into each bioreactor. This design was translated into a multi-layer chip prototype and a routine manufacturing procedure was established. The first series of microscopable, chemically resistant and sterilizable chip prototypes was tested for matrix compatibility and primary cell culture suitability. Sterility and long-term human cell survival could be shown. Optimizing the applied design approach and prototyping tools resulted in a time period of only 3 months for a single design and prototyping cycle. This rapid prototyping scheme now allows for fast adjustment or redesign of inaccurate architectures. The designed chip platform is thus ready to be evaluated for the establishment and maintenance of the human liver, brain cortex and bone marrow micro-organoids in a systemic microenvironment.

Keywords
Micro-system technologies; Micro-fluidics; Organ-on-a-chip; Micro-organoid; Multi-micro-organ bioreactor
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Design and prototyping of a chip-based multi-micro-organoid culture system for substance testing, predictive to human (substance) exposure
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 148, Issue 1, 1 July 2010, Pages 70–75
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us