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Cis-regulatory elements affecting the Nanos gene promoter in the germline stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24372 43510 2010 7 PDF Available
Title
Cis-regulatory elements affecting the Nanos gene promoter in the germline stem cells
Abstract

Drosophila Nanos gene plays an important role in stem cell maintenance and body patterning. With the purpose of understanding the cis-regulatory machinery involved in the transcription of the nanos gene in the germline stem cells, we examined its promoter fragment from +97 to −708 relative to the transcription start site and identified enhancer elements located between position −108 and +97. Experiments with transgenic flies revealed that the minimal promoter (from −108 to +20) is sufficient in the germline stem cells for the GFP expression in transgenic Drosophila. Moreover, the flag-tagged nanos protein blotting experiments revealed that a short promoter fragment plus some sequences of the nos 5′ UTR spanning −108 to +97 could efficiently drive the expression of the flag-tagged [Nos-mRNA-nos3′UTR] transgene in transgenic flies indicating that the cis-regulatory elements located between positions −108 and +97 of the nanos promoter are sufficient to fully transcribe the nanos mRNA. Deletion of the identified cis-acting sequences from the promoter rendered it non-functional as it could no longer transcribe the nanos mRNA in transgenic flies thus revealing the importance of these sequences for the transcription of the nanos gene.

Keywords
Cis-acting elements; Germline stem cells; Nos promoter
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Cis-regulatory elements affecting the Nanos gene promoter in the germline stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 4, 15 February 2010, Pages 323–329
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us