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Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone—Development, scope and limitations

Paper ID Volume ID Publish Year Pages File Format Full-Text
24377 43510 2010 8 PDF Available
Title
Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone—Development, scope and limitations
Abstract

5-O-Coumarinyl-d-xylulose was studied as a fluorogenic substrate for the stereospecific assay of transketolase enzyme. Enzymatic C2–C3 cleavage released an α-hydroxyl, β-coumarinyl substituted aldehyde. Although the subsequent β-elimination step was rate limiting under chemical or enzymatic catalysis, we detected a TK activity as low as 0.7 mIU. To improve the fluorescence signal release, kinetic and product distribution analyses of this reaction were performed by LC/UV/MS coupling.

Keywords
Biocatalysis; Fluorogenic substrate; β-Elimination; High-throughput screening; Transketolase; C–C bond
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Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone—Development, scope and limitations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 4, 15 February 2010, Pages 359–366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us