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Recombinant rabies virus glycoprotein synthesis in bioreactor by transfected Drosophila melanogaster S2 cells carrying a constitutive or an inducible promoter

Paper ID Volume ID Publish Year Pages File Format Full-Text
24385 43511 2010 4 PDF Available
Title
Recombinant rabies virus glycoprotein synthesis in bioreactor by transfected Drosophila melanogaster S2 cells carrying a constitutive or an inducible promoter
Abstract

S2 cell populations (S2AcRVGP2K and S2MtRVGP-Hy) were selected after transfection of gene expression vectors carrying the cDNA encoding the rabies virus glycoprotein (RVGP) gene under the control of the constitutive (actin) or inductive (metallothionein) promoters. These cell populations were cultivated in a 1 L bioreactor mimicking a large scale bioprocess. Cell cultures were carried out at 90 rpm and monitored/controlled for temperature (28 °C) and dissolved oxygen (10 or 50% air saturation). Cell growth attained ∼1.5–3 × 107 cells/mL after 3–4 days of cultivation. The constitutive synthesis of RVGP in S2AcRVGP2K cells led to values of 0.76 μg/107 cells at day 4 of culture. The RVGP synthesis in S2MtRVGP-Hy cell fraction increased upon CuSO4 induction attaining specific productivities of 1.5–2 μg/107 cells at days 4–5. RVGP values in supernatant as a result of cell lysis were always very low (<0.2 μg/mL) indicating good integrity of cells in culture. Overall the RVGP productivity was of 1.5–3 mg/L. Our data showed an important influence of dissolved oxygen on RVGP synthesis allowing a higher and sustained productivity by S2MtRVGP-Hy cells when cultivated with a DO of 10% air saturation. The RVGP productivity in bioreactors shown here mirrors those previously observed for T-flasks and shaker bottles and allow the preparation of the large RVGP quantities required for studies of structure and function.

Keywords
Drosophila melanogaster S2 cell; Rabies viral glycoprotein (RVGP); Constitutive/inducible promoter; Bioprocess; Bioreactor
First Page Preview
Recombinant rabies virus glycoprotein synthesis in bioreactor by transfected Drosophila melanogaster S2 cells carrying a constitutive or an inducible promoter
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 146, Issue 4, 15 April 2010, Pages 169–172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering