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Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system

Paper ID Volume ID Publish Year Pages File Format Full-Text
24389 43511 2010 4 PDF Available
Title
Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system
Abstract

Bioreactor systems have been developed as alternatives to standard culture flasks due to their homogeneous nature, easiness of monitoring and increased cell production. Here we investigated the in vitro expansion of bone marrow (BM) mesenchymal stem cells (MSC) in spinner flasks, using gelatin microcarriers (Cultispher S) to support cell adhesion and proliferation. MSC expansion was performed using a low-serum containing medium (2% of fetal bovine serum, FBS).A strategy was defined for the maximization of cell expansion: microcarriers were pre-coated with FBS in order to increase cell seeding efficiency and an adequate feeding regime was established (25% medium exchange everyday). The maximum cell density, 4.2 × 105 cells/mL, was obtained at day 8, corresponding to a fold increase in total cell number of 8.4 ± 0.8. Expanded MSC retained their differentiation potential into adipogenic and osteogenic lineages, as well as their clonogenic ability. Harvested cells expressed >90% of CD73, CD90 and CD105 markers.These results demonstrated that a microcarrier-based stirred culture system is adequate for human MSC expansion, using a low-serum containing medium, allowing the generation of significant cell numbers for potential applications in regenerative medicine.

Keywords
Mesenchymal stem cells; Expansion; Microcarriers; Spinner flask; Low-serum content medium; Cell metabolism
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Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 146, Issue 4, 15 April 2010, Pages 194–197
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us