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High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process

Paper ID Volume ID Publish Year Pages File Format Full-Text
24399 43512 2010 6 PDF Available
Title
High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process
Abstract

A process comprising two-step fed-batch cultivation has been investigated for the production of ectoines using the halophilic bacterium Halomonas boliviensis DSM 15516T. The first cultivation was performed under optimal conditions for cell growth and resulted in cell mass concentration of about 41 g l−1 after 24 h of cultivation. During the second cultivation at higher salt concentration, accumulation of ectoines increased while cell mass decreased with increasing salt concentration. Maximum productivity of total ectoines reached was 10 g l−1 d−1 with ectoine concentration of 6 g l−1 and hydroxyectoine concentration of 8 g l−1 after 9 h of cultivation at 18.5% NaCl, which is among the highest reported so far. H. boliviensis cells were further recycled for the production process after releasing the ectoines. About 75% of the accumulated ectoines were released by subjecting the cells to hypoosmotic shock. On subsequent reincubation in a medium containing higher salt concentration the cells were able to re-synthesize the ectoines resulting in a global productivity of 11.1 g l−1 d−1, and ectoine and hydroxyectoine productivities of 9.1 g l−1 d−1 and 2.0 g l−1 d−1, respectively.

Keywords
Fed-batch cultivation; Ectoine; Hydroxyectoine; Halomonas boliviensis
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High productivity of ectoines by Halomonas boliviensis using a combined two-step fed-batch culture and milking process
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 147, Issue 1, 3 May 2010, Pages 46–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us