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Folding reporter tags can deliver misleading results upon chaperone coexpression

Paper ID Volume ID Publish Year Pages File Format Full-Text
24407 43513 2009 4 PDF Available
Title
Folding reporter tags can deliver misleading results upon chaperone coexpression
Abstract

Chaperone coexpression is a common strategy for improving the folding quality of overexpressed proteins. Tags with quantifiable biological properties are often fused to the target protein as indicators of functional expression. Using a fusion of the enzymes AtCCD1 and GST, we show that chaperone coexpression can alter the folding quality of fusion protein moieties dissimilarly, sometimes even in opposite directions. Incorrect results can be obtained if target protein folding quality is assessed solely based on reporter tag activity. Thus, additional methods for the verification of the obtained results should be performed.

Keywords
Chaperone; Fusion protein; Oxidoreductases; Protein folding; Protein modifications; Reporter tag
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Folding reporter tags can deliver misleading results upon chaperone coexpression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 144, Issue 4, December 2009, Pages 268–271
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us