fulltext.study @t Gmail

An evaluation framework for statistical tests on microarray data

Paper ID Volume ID Publish Year Pages File Format Full-Text
24423 43514 2009 9 PDF Available
Title
An evaluation framework for statistical tests on microarray data
Abstract

Microarray analysis has become a popular and routine method in functional genomics. It is typical for such experiments to involve a small number of replicates, which causes unreliable estimates of the sample variance. Microarrays have fostered the development of new statistical methods to analyze data resulting from experiments with small sample sizes. In this study, we tackle the problem of evaluating the performance of statistical tests for generating ranked gene lists from two-channel direct comparisons. We propose an evaluation method based on a oligonucleotide microarray with a large number of replicate spots yielding a maximum of 400 replicates per gene. We apply Spearman’s rank correlation coefficient to ranked gene-lists generated by eight widely used microarray specific test statistics, which are applied to small random samples. We could show that variance stabilizing methods such as Cyber-T, SAM, and LIMMA can be beneficial for very small sample sizes and that SAM and the t-test provide stronger control of the type I error rate than the other methods. Specifically, we report that for four replicates all methods reach a high to very high correlation with our reference standard.

Keywords
Microarray; Statistical test; Inference; Evaluation; Comparative study
First Page Preview
An evaluation framework for statistical tests on microarray data
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 140, Issues 1–2, 10 March 2009, Pages 18–26
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us