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On-line estimation of biomass, glucose and ethanol in Saccharomyces cerevisiae cultivations using in-situ multi-wavelength fluorescence and software sensors

Paper ID Volume ID Publish Year Pages File Format Full-Text
24443 43515 2009 11 PDF Available
Title
On-line estimation of biomass, glucose and ethanol in Saccharomyces cerevisiae cultivations using in-situ multi-wavelength fluorescence and software sensors
Abstract

Batch bioreactor cultivations using Saccharomyces cerevisiae at high (190–305 g l−1 glucose) or low (21–25 g l−1 glucose) gravity conditions were monitored on-line using multi-wavelength fluorescence (MWF) and standard monitoring sensors. Partial least squares models were calibrated for the prediction of cell dry weight (CDW), ethanol and consumed glucose, using the two data types separately. The low gravity cultivations (LGCs) consisted of two phases (glucose consumption with concomitant ethanol production followed by ethanol consumption after glucose depletion), which proved difficult to model using one and the same model for both phases. Segmented modelling, using different models for the two phases, improved the predictions significantly. The prediction models calibrated on standard on-line process data displayed similar or lower root mean square error of prediction (RMSEP) compared to the fluorescence models. The best prediction models for high gravity cultivations (HGCs) had RMSEPs of 1.0 g l−1 CDW, 1.8 g l−1 ethanol and 5.0 g l−1 consumed glucose, corresponding to 4%, 2% and 2% of the respective concentration intervals. Corresponding numbers in low gravity models were 0.3 g l−1 CDW, 0.7 g l−1 ethanol and 1.0 g l−1 consumed glucose, corresponding to 4%, 8% and 4% of the respective concentration intervals.

Keywords
HGC, high gravity cultivation; LGC, low gravity cultivation; MWF, multi-wavelength fluorescence; μ, specific growth rate (h−1); μi, specific growth rate during growth on substrate i (h−1); Yi/j, yield of species i per unit of species j (cmol cmol−1)Biopro
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On-line estimation of biomass, glucose and ethanol in Saccharomyces cerevisiae cultivations using in-situ multi-wavelength fluorescence and software sensors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 144, Issue 2, 26 October 2009, Pages 102–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us