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Production of l-lactate in Leuconostoc citreum via heterologous expression of l-lactate dehydrogenase gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
24450 43515 2009 5 PDF Available
Title
Production of l-lactate in Leuconostoc citreum via heterologous expression of l-lactate dehydrogenase gene
Abstract

d-form lactate is often found in fermented foods and excessive dietary intake of d-lactate may cause metabolic stress in both infants and patients. Leuconostoc citreum is a major lactic acid bacterium that produces d-lactate in fermented foods. The aim of this study was to change the pyruvate carbon flux in L. citreum from d-lactate into l-lactate by heterologous expression of l-lactate dehydrogenase (ldhL) gene. For this, ldhL from Lactobacillus plantarum was cloned and introduced into L. citreum using a shuttle vector pLeuCM. In the transformant, ldhL was successfully transcribed and l-lactate dehydrogenase was expressed. As a consequence of transformation, the ratio between d- and l-isomers was changed due to the increment of l-lactate and the decrement of d-lactate, but no significant differences were found in total lactate concentration between the host and transformant cells. This is the first report of metabolic engineering in Leuconostoc by modulating the central carbon flux into health-favored way.

Keywords
Leuconostoc citreum; l-/d-Lactate; l-/d-Lactate dehydrogenase; Gene transformation
First Page Preview
Production of l-lactate in Leuconostoc citreum via heterologous expression of l-lactate dehydrogenase gene
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 144, Issue 2, 26 October 2009, Pages 160–164
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering