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Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii

Paper ID Volume ID Publish Year Pages File Format Full-Text
24456 43516 2010 6 PDF Available
Title
Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii
Abstract

The coding region of both the ferrochelatase gene, hemH, from Bradyrhizobium japonicum, and the leghemoglobin gene, lba, from Glycine max, were transferred into chloroplast of Chlamydomonas reinhardtii. As a result, transgenic C. reinhardtii cultures more rapidly consumed O2 and increased H2 output compared with controls in both sulfur-free and sulfur-containing medium. H2 production of the transgenic algal cultures in sulfur-free medium was 4-fold greater than that of control cultures, ∼3.3 ml bottle−1. Maximum expression of the hemH-lba fusion protein on day 5 coincided with the lowest levels of O2 content and the highest H2 evolution rate detected over 7 days of anaerobic induction in sulfur-free medium. When the concentration of sulfate in the growth medium was restored to 12.5 or 50 μM, O2 consumption and H2 yield decreased more slowly in the transgenic algal cultures than in the control cultures. These results demonstrate that expression of the hemH-lba fusion protein in chloroplast of C. reinhardtii improved their H2 yield by decreasing O2 content in the medium, thereby representing the potential for H2 production in green algae to be improved.

Keywords
PSII, photosystem II; Spec, spectinomycin; WT, wild type; lb, leghemoglobin; S, sulfur; ORF, open reading frame; ALA, δ-aminolevulinic acid; Fe, ferro; MM, molecular markerChlamydomonas reinhardtii; Leghemoglobin; Ferrochelatase; Bio-hydrogen production;
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Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 146, Issue 3, 1 April 2010, Pages 120–125
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us