fulltext.study @t Gmail

Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
24471 43517 2009 6 PDF Available
Title
Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene
Abstract

For biotechnological production of coenzyme Q10 (CoQ10) in recombinant Escherichia coli, three genetic manipulations were performed: heterologous expression of decaprenyl diphosphate synthase (Dps) from Agrobacterium tumefaciens, deletion of endogenous octaprenyl diphosphate synthase (IspB), and overexpression of 1-deoxy-d-xylulose synthase (Dxs). Expression of the dps gene and deletion of the ispB gene in E. coli BL21(DE3)ΔispB/pAP1 allowed production of CoQ10 only. Furthermore, coexpression of the dxs gene increased the specific content of CoQ10 from 0.55–0.89 mg g−1 to 1.40 mg g−1. For mass production of CoQ10, fed-batch fermentation of E. coli BL21(DE3)ΔispB/pAP1 + pDXS was carried out in a defined medium with 20 g l−1 initial glucose and by the glucose-feeding strategy of pH-stat. Finally, 99.4 mg l−1 CoQ10 concentration, 1.41 mg g−1 specific CoQ10 content and 3.11 mg l−1 h−1 productivity were obtained in 33 h of the fermentation, which were 78, 1.9, and 19 times higher than those for E. coli BL21(DE3)/pAP1 without the ispB deletion and dxs overexpression.

Keywords
Coenzyme Q10; Escherichia coli; Decaprenyl diphosphate synthase; Octaprenly diphosphate synthase; 1-Deoxy-d-xylulose synthase; Fed-batch fermentation
First Page Preview
Synergistic effects of chromosomal ispB deletion and dxs overexpression on coenzyme Q10 production in recombinant Escherichia coli expressing Agrobacterium tumefaciens dps gene
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 144, Issue 1, 12 October 2009, Pages 64–69
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us