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Enhancement of protein transduction-mediated nuclear delivery by interaction with dynein/microtubules

Paper ID Volume ID Publish Year Pages File Format Full-Text
24475 43518 2010 4 PDF Available
Title
Enhancement of protein transduction-mediated nuclear delivery by interaction with dynein/microtubules
Abstract

Nucleocytoplasmic trafficking is a major consideration for the design of vehicles for the delivery of drug/DNA cargo to cell nuclei for cancer and gene therapies. Recent data indicate that efficient nuclear import can involve the microtubule (MT)/dynein network, such that nuclear delivery of exogenous cargo could be enhanced by attachment to peptide modules mediating association with dynein components, but this has not been investigated. Here, we report that the nuclear delivery of an exogenous cargo that enters the cell by protein transduction can be enhanced by attachment to a dynein-association sequence, with dependence on the MT network. This indicates that dynein/MT-association modules may provide useful modules for DNA/drug delivery approaches.

Keywords
Dynein; Microtubules; Nuclear import; Cell penetrating peptide; Protein transduction
First Page Preview
Enhancement of protein transduction-mediated nuclear delivery by interaction with dynein/microtubules
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 3, 1 February 2010, Pages 222–225
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering