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Design of a novel Couette flow bioreactor to study the growth of fungal microorganism

Paper ID Volume ID Publish Year Pages File Format Full-Text
24481 43518 2010 9 PDF Available
Title
Design of a novel Couette flow bioreactor to study the growth of fungal microorganism
Abstract

Cultivations using Trichoderma reesei Rut C-30 were performed in a 5-l Couette flow bioreactor (CFB) which was designed and built to perform experiments in batch and continuous modes. Process parameters such as dissolved oxygen, pH and temperature were measured and controlled without disturbing the shear profile inside the bioreactor. Effect of shear on the growth, protein production and morphology was studied by performing runs at 100, 200, 300 and 400 rpm. At higher shear rates, lower protein production rate and activity, and higher rate of fragmentation were observed. Also, the cell thickness decreased with increasing speed, going from 8.3 μm for the experiment at 100 rpm to 4.3 μm at 400 rpm. The effect of substrate, lactose (an inducer) or glucose, was investigated by switching the feed medium during the two runs performed at 300 and 400 rpm. The novel design of the CFB used in the present study includes a large volume that allows growing larger size microorganisms (e.g. fungi) and permits larger sampling volumes without affecting the cultivation. It also has the ability to carry out experiments for long periods of time, both in batch and continuous modes.

Keywords
Cellulase; Couette flow bioreactor; Morphology; Shear; Trichoderma reesei
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Design of a novel Couette flow bioreactor to study the growth of fungal microorganism
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 3, 1 February 2010, Pages 264–272
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us