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Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Paper ID Volume ID Publish Year Pages File Format Full-Text
24490 43519 2009 3 PDF Available
Title
Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector
Abstract

A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.

Keywords
BGM, Bacillus subtilis genome; BAC, bacterial artificial chromosome; bp, base pair(s); kb, kilo bases; Mb, mega bases; spc, spectinomycin-resistance gene; cI, cI repressor gene; bsr, blasticidin S-resistance gene; neo, neomycin phosphotransferase gene; Nm
First Page Preview
Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 3, 5 February 2009, Pages 211–213
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering