fulltext.study @t Gmail

The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR

Paper ID Volume ID Publish Year Pages File Format Full-Text
24491 43519 2009 8 PDF Available
Title
The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR
Abstract

Serine hydroxymethyltransferase (SHMT) occupies a central position in one-carbon metabolism, and we here study its regulation in Corynebacterium glutamicum. Enzyme quantifications revealed an about 3-fold increase of SHMT activity during exponential growth with a further increase at the onset of the stationary phase. The SHMT encoding glyA gene was shown to be transcribed as a monocistronic mRNA, and its transcriptional start site was determined. Using DNA affinity chromatography the regulator GlyR (Cg0527) was identified and its chromosomal deletion shown to abolish the increase in SHMT activity in the stationary phase. The involvement of GlyR in glyA control was further confirmed by a transcriptional fusion of the glyA promoter with cat and chloramphenicol acetyltransferase quantifications. GlyR was isolated and mutational studies together with electrophoretic mobility shift assays showed that it binds to the imperfect palindromic motif CACT-N2-AATG in the −119 to −96 upstream region of the glyA promoter. These and further data illustrate that the essential SHMT has highest activity in the stationary phase and that GlyR acts as an activator of glyA transcription in this growth phase.

Keywords
glyA; SHMT; Stationary phase; Regulation; ArsR-type regulator
First Page Preview
The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 3, 5 February 2009, Pages 214–221
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us