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Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
24492 43519 2009 7 PDF Available
Title
Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells
Abstract

The generation of nitric oxide (NO) in Taxus cuspidata in immobilized support matrices and the potential role of NO as signal molecular in regulation of Taxol production were investigated. It was found that the immobilization induced a spatial and temporal-dependent NO burst in immobilized supported matrices. NO level reached the maximum in the central zone of immobilized supported matrices on day 20, which was more than twice compared with that in suspended cells. Further investigations showed that the phenylalanine ammonialyase (PAL) activity and Taxol production of the 20-day-old immobilized T. cuspidata cells increased by onefold and 11% after 4 h treatment with 20 μM NO donor (sodium nitroprusside), respectively. NO inhibitor Nω-nitro-l-arginine and NO scavenger 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde partially blocked PAL activity and Taxol accumulation in immobilized cells. These results suggest that NO plays a signal role in regulation of PAL activity and Taxol production in immobilized T. cuspidata cells.

Keywords
Nitric oxide; Immobilization; Phenylalanine ammonialyase; Taxol; Taxus cuspidata
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Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 3, 5 February 2009, Pages 222–228
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us