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Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail

Paper ID Volume ID Publish Year Pages File Format Full-Text
24493 43519 2009 7 PDF Available
Title
Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail
Abstract

A β-d-glycosidase (G I) from the China white jade snail showed non-Michaelis–Menten mode in catalyzing the reaction using pNPGlu and pNPFuc as the substrate and monitoring the released pNP. We determined quantitatively both the transglycosidic and hydrolytic products of pNPGlu and pNPFuc solvolysis for the detailed kinetic analysis on G I-catalyzed hydrolysis and transglycosylation reaction. The inhibition kinetic studies using deoxynojirimycin (DNJ) and butanol as inhibitors were preceded. DNJ only inhibited competitively the hydrolysis of cellobiose and pNPGlu while “activated” the transglycosylation of pNPGlu and pNPFuc. This was evident from the increased Vmaxtr value with no change of the apparent Kmtr. In contrast, butanol exhibited a competitive inhibition to the transglycosylation reaction and non-competitive inhibition to the hydrolysis. The results indicated that the non-Michaelis–Menten kinetic behavior was caused by the co-occurrence of substrate transglycosylation reaction. This study provided a simple method to increase the transglycosylation yield by using DNJ to inhibit hydrolysis.

Keywords
β-d-Glycosidase; Transglycosylation activity; Kinetics; Allosteric effect; The China white jade snail
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Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 3, 5 February 2009, Pages 229–235
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us