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Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
24494 43519 2009 14 PDF Available
Title
Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies
Abstract

A system for vascular hollow fiber bio-artificial pancreas development, optimization and in vitro testing was implemented and operated in a simple and fully described manner, allowing other researchers to test a variety of experimental conditions (different biomaterials, biologic tissue, addition of proteins or other adjuvants). In this work, a polysulfone hollow fiber was used as bioprotective material. Two different cell sources were co-immobilized with agarose microspheres in and experimented with the membrane device: rat islets of Langerhans and mouse β-TC-3 insulinoma cells. The results obtained with islets of Langerhans were used as islet comparable insulin-release data. Beta-TC-3 cells were mainly used in these studies, due to higher control and reproducibility of cell number and behavior: addition of hemoglobin was beneficial for sustained cell viability, especially during cell insertion in the device (viability assessed by β-TC-3 lactate dehydrogenase activity in the recirculating culture medium); cells did not adhere to the polysulfone membrane (assessed by SEM observation of membrane samples from dynamic cultures). Comparable device functionality and insulin-release results were attained with both cell types: device functionality was maintained for 7–9 days and maximum insulin-release during dynamic glucose challenges were 2.6 × 10−3 ± 7 × 10−5 μU/β-cell × 8 h, with islets, and 9.3 × 10−4 ± 2 × 10−5 μU/β-cell × 2 h, with β-TC-3 cells.

Keywords
Artificial organs; Bio-artificial pancreas; Hollow fibers in vascular device; Islets of Langerhans; β-TC-3 cells; BAP-in vitro assays
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Development of a polysulfone hollow fiber vascular bio-artificial pancreas device for in vitro studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 139, Issue 3, 5 February 2009, Pages 236–249
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us