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Monooxygenases as biocatalysts: Classification, mechanistic aspects and biotechnological applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
24508 43521 2010 16 PDF Available
Title
Monooxygenases as biocatalysts: Classification, mechanistic aspects and biotechnological applications
Abstract

Monooxygenases are enzymes that catalyze the insertion of a single oxygen atom from O2 into an organic substrate. In order to carry out this type of reaction, these enzymes need to activate molecular oxygen to overcome its spin-forbidden reaction with the organic substrate. In most cases, monooxygenases utilize (in)organic cofactors to transfer electrons to molecular oxygen for its activation. Monooxygenases typically are highly chemo-, regio-, and/or enantioselective, making them attractive biocatalysts. In this review, an exclusive overview of known monooxygenases is presented, based on the type of cofactor that these enzymes require. This includes not only the cytochrome P450 and flavin-dependent monooxygenases, but also enzymes that utilize pterin, metal ions (copper or iron) or no cofactor at all. As most of these monooxygenases require nicotinamide coenzymes as electron donors, also an overview of current methods for coenzyme regeneration is given. This latter overview is of relevance for the biotechnological applications of these oxidative enzymes.

Keywords
Biocatalysis; Coenzyme regeneration; Cofactors; Copper-dependent monooxygenases; Flavin-dependent monooxygenases; Non-heme iron-dependent monooxygenases; P450 monooxygenases; Pterin monooxygenases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 146, Issues 1–2, March 2010, Pages 9–24
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us