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The GlxR regulon of the amino acid producer Corynebacteriumglutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model

Paper ID Volume ID Publish Year Pages File Format Full-Text
24519 43522 2009 8 PDF Available
Title
The GlxR regulon of the amino acid producer Corynebacteriumglutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model
Abstract

Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C. glutamicum ATCC 13032 encodes a DNA binding transcription factor of the Crp-Fnr protein family. Available data indicated a prominent role of GlxR in the transcriptional regulatory network of C. glutamicum. We have used recently published whole-genome annotations of several corynebacteria to derive further evidence for GlxR-mediated transcriptional regulation from a comparative genomics approach. A bioinformatic strategy detected 22 genes belonging to a proposed GlxR core regulon in corynebacteria. Binding of purified GlxR protein to 40-mer oligonucleotides representing predicted GlxR binding sites in the upstream region of core genes from C. glutamicum ATCC 13032 was verified in vitro by electrophoretic mobility shift assays. Based on current data, a reliable genome-scale prediction of GlxR binding sites was performed, indicating that about 14% of the annotated C. glutamicum genes might be under direct transcriptional control by GlxR. Integration of GlxR-mediated regulatory interactions with the data stored in CoryneRegNet enabled the reconstruction of a refined transcriptional regulatory network model for C. glutamicum. This network model exhibited a hierarchical and modular structure and is characterized by the presence of master regulators in each functional module, with GlxR serving as the dominating regulatory hub.

Keywords
Corynebacterium glutamicum; Comparative genomics; Transcriptional regulatory network; Transcription factor binding site; Global regulator; GlxR
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The GlxR regulon of the amino acid producer Corynebacteriumglutamicum: Detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 143, Issue 4, 25 September 2009, Pages 239–246
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us