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Enhanced yield of medium-chain-length polyhydroxyalkanoates from nonanoic acid by co-feeding glucose in carbon-limited, fed-batch culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
24522 43522 2009 6 PDF Available
Title
Enhanced yield of medium-chain-length polyhydroxyalkanoates from nonanoic acid by co-feeding glucose in carbon-limited, fed-batch culture
Abstract

Medium-chain-length polyhydroxyalkanoates (MCL-PHAs) were produced in carbon-limited, single-stage, fed-batch fermentations of Pseudomonas putida KT2440 by co-feeding nonanoic acid (NA) and glucose (G) to enhance the yield of PHA from NA. An exponential (μ = 0.25 h−1) followed by a linear feeding strategy at a NA:G ratio of 1:1 (w/w) achieved 71 g l−1 biomass containing 56% PHA. Although the same overall PHA productivity (1.44 g l−1 h−1) was obtained when NA alone was fed at the same specific growth rate, the overall yield of PHA from NA increased by 25% (0.66 g PHA g NA−1 versus 0.53 g g−1) with glucose co-feeding. Further increasing glucose in the feed (NA:G = 1:1.5) resulted in a slightly higher yield (0.69 g PHA g NA−1) but lower PHA content (48%) and productivity (1.16 g l−1 h−1). There was very little change in the PHA composition.

Keywords
Medium-chain-length; Polyhydroxyalkanoate; Fed-batch; Fermentation; Pseudomonas putida; PHA; MCL-PHA
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Enhanced yield of medium-chain-length polyhydroxyalkanoates from nonanoic acid by co-feeding glucose in carbon-limited, fed-batch culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 143, Issue 4, 25 September 2009, Pages 262–267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us