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Flow cytometric and morphological analyses of Pinus pinaster somatic embryogenesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
24526 43522 2009 8 PDF Available
Title
Flow cytometric and morphological analyses of Pinus pinaster somatic embryogenesis
Abstract

An approach combining morphological profiling and flow cytometric analysis was used to assess genetic stability during the several steps of somatic embryogenesis in Pinus pinaster. Embryogenic cell lines of P. pinaster were established from immature zygotic embryos excised from seeds obtained from open-pollinated trees. During the maturation stage, phenotype of somatic embryos was characterized as being either normal or abnormal. Based upon the prevalent morphological traits, different types of abnormal embryos underwent further classification and quantification. Nuclear DNA content of maritime pine using the zygotic embryos was estimated to be 57.04 pg/2C, using propidium iodide flow cytometry. According to the same methodology, no significant differences (P ≤ 0.01) in DNA ploidy were detected among the most frequently observed abnormal phenotypes, embryogenic cell lines, zygotic and normal somatic embryos, and somatic embryogenesis-derived plantlets. Although the differences in DNA ploidy level do not exclude the occurrence of a low level of aneuploidy, the results obtained point to the absence of major changes in ploidy level during the somatic embryogenesis process of this economically important species. Therefore, our primary goal of true-to-typeness was assured at this level.

Keywords
EB, ethidium bromide; ECLs, embryogenic cell lines; FCM, flow cytometry; PI, propidium iodide; SeII, pre-cotyledonary somatic embryo; SeIII, cotyledonary somatic embryo; SE, somatic embryogenesisDNA ploidy level; Flow cytometry; Nuclear DNA content; Pinus
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Flow cytometric and morphological analyses of Pinus pinaster somatic embryogenesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 143, Issue 4, 25 September 2009, Pages 288–295
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us