fulltext.study @t Gmail

The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla)

Paper ID Volume ID Publish Year Pages File Format Full-Text
24533 43524 2008 5 PDF Available
Title
The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla)
Abstract

European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3′,4,4′-tetrachlorobiyphenyl (PCB77) to examine and characterize the inductive effect of coplanar PCBs on CYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 injection on transcription activity of the housekeeping gene glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) was tested to determine its suitability as a reference gene for further quantitative gene expression analyses. Our results clearly indicate a significant dose-dependent increase in CYP1A1 gene expression in the gills of European eel, while in liver tissues a significant elevation in CYP1A1 gene expression was only detectable at highest contamination rates, indicating the potential of CYP1A1 differential gene expression analysis in gills as a biomarker for PCB contamination in eels. PCB77 contamination did not affect GAPDH transcription in gills but, at highest doses, resulted in a significant elevation in liver, speaking against GAPDH as a reference housekeeping gene after PCB exposure.

Keywords
European eel; PCBs; Gene expression; Cytochrome p450; Biomarker
First Page Preview
The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla)
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 136, Issues 3–4, 10 September 2008, Pages 135–139
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us