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The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing

Paper ID Volume ID Publish Year Pages File Format Full-Text
24555 43526 2008 11 PDF Available
Title
The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing
Abstract

Corynebacterium urealyticum is a lipid-requiring, urealytic bacterium of the human skin flora that has been recognized as causative agent of urinary tract infections. We report the analysis of the complete genome sequence of C. urealyticum DSM7109, which was initially recovered from a patient with alkaline-encrusted cystitis. The genome sequence was determined by a combination of pyrosequencing and Sanger technology. The chromosome of C. urealyticum DSM7109 has a size of 2,369,219 bp and contains 2024 predicted coding sequences, of which 78% were considered as orthologous with genes in the Corynebacterium jeikeium K411 genome. Metabolic analysis of the lipid-requiring phenotype revealed the absence of a fatty acid synthase gene and the presence of a β-oxidation pathway along with a large repertoire of auxillary genes for the degradation of exogenous fatty acids. A urease locus with the gene order ureABCEFGD may play a pivotal role in virulence of C. urealyticum by the alkalinization of human urine and the formation of struvite stones. Multidrug resistance of C. urealyticum DSM7109 is mediated by transposable elements, conferring resistances to macrolides, lincosamides, ketolides, aminoglycosides, chloramphenicol, and tetracycline. The complete genome sequence of C. urealyticum revealed a detailed picture of the lifestyle of this opportunistic human pathogen.

Keywords
Corynebacterium urealyticum; Genome sequence; β-Oxidation; Virulence factors; Urinary tract infection; Antibiotic resistance
First Page Preview
The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 136, Issues 1–2, 31 August 2008, Pages 11–21
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering