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Quantitative considerations for suspension array assays

Paper ID Volume ID Publish Year Pages File Format Full-Text
24567 43527 2010 6 PDF Available
Title
Quantitative considerations for suspension array assays
Abstract

A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 108 copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoretical framework. The assay was also evaluated on RNA extracts taken from stimulated macrophages, to demonstrate that changes in gene expression can be accurately monitored in a biological system. We believe the reproducibility and accuracy of the assay presented herein will be useful for high-throughput, multiplex sensitive detection of nucleic acids over a large dynamic range in a research or diagnostic setting.

Keywords
Suspension arrays; DNA hybridisation; DNA quantification; Microbeads; Flow cytometry; Immobilised oligonucleotides
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Quantitative considerations for suspension array assays
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 1, 1 January 2010, Pages 17–22
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us