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An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available

Paper ID Volume ID Publish Year Pages File Format Full-Text
24571 43527 2010 4 PDF Available
Title
An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available
Abstract

Although the cost of high throughput sequencing is decreasing, the cost is still often too high for individual projects targeted at, e.g., genome-wide transcription profiling in non-model organisms. Then, a low-cost alternative is cDNA-AFLP, which we have now considerably modified in order to develop a faster and simpler method to identify and analyze genes involved in specific, possibly adaptive characteristics. Particularly, we wanted to exclude repetitive PCR amplifications, extensive cloning and the presence of overlapping transcripts, which all lower the efficiency of the method.

Keywords
cDNA-AFLP fingerprinting; Transcription profiling; Adaptive markers; Non-model organisms
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An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 1, 1 January 2010, Pages 43–46
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us