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Specific growth rate dependent transcriptome profiling of Escherichia coli K12 MG1655 in accelerostat cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
24574 43527 2010 6 PDF Available
Title
Specific growth rate dependent transcriptome profiling of Escherichia coli K12 MG1655 in accelerostat cultures
Abstract

Specific growth rate dependent gene expression changes of Escherichia coli K12 MG1655 were studied by microarray and real-time PCR analyses. The bacteria were cultivated on glucose limited minimal medium using the accelerostat method (A-stat) where starting from steady state conditions (chemostat culture) dilution rate is constantly increased. At specific growth rate (μ) 0.47 h−1, E. coli had focused its metabolism to glucose utilization by down-regulation of alternative substrate transporters expression compared to μ = 0.3 h−1. It was found that acetic acid accumulation began at μ = 0.34 ± 0.01 h−1 and two acetate synthesis pathways – phosphotransacetylase-acetate kinase (pta-ackA) and pyruvate oxidase (poxB) – contributed to the synthesis at the beginning of overflow metabolism, i.e. onset of acetate excretion. On the other hand, poxB, pta and ackA expression patterns suggest that pyruvate oxidase may be the only enzyme synthesizing acetate at μ = 0.47 h−1. Loss of glucose and acetate co-utilization represented by down-regulation of acs-yjcH-actP operon between specific growth rates 0.3–0.42 h−1 and acetic acid accumulation from μ = 0.34 ± 0.01 h−1 allows one to surmise that the acetate utilization operon expression might play an important role in overflow metabolism.

Keywords
A-stat; Accelerostat; Continuous culture; Escherichia coli; Overflow metabolism; Microarray
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Specific growth rate dependent transcriptome profiling of Escherichia coli K12 MG1655 in accelerostat cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 145, Issue 1, 1 January 2010, Pages 60–65
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us