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Transcriptional activation increases RNA silencing efficiency and stability in the fungus Mucor circinelloides

Paper ID Volume ID Publish Year Pages File Format Full-Text
24583 43528 2009 4 PDF Available
Title
Transcriptional activation increases RNA silencing efficiency and stability in the fungus Mucor circinelloides
Abstract

Initiation and maintenance of the RNA silencing mechanism was investigated after transcriptional activation of the transgene carB in Mucor circinelloides. Light induced transcription of the reporter gene carB specifically increased the 21-nt siRNA compared with the 25-nt siRNA and rose the silencing maintenance from 48% up to 93% of the descendent colonies. In accordance, induced transcription of the gene carB through disruption of the gene crgA increased the initial silencing frequency up to 30-fold, when compared with the frequency of silencing obtained using a crgA+ genetic background.

Keywords
RNA silencing; Mucor circinelloides; SiRNA; CrgA
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Transcriptional activation increases RNA silencing efficiency and stability in the fungus Mucor circinelloides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 142, Issue 2, 15 June 2009, Pages 123–126
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us