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Identification of critical infection parameters to control helper-dependent adenoviral vector production

Paper ID Volume ID Publish Year Pages File Format Full-Text
24586 43528 2009 9 PDF Available
Title
Identification of critical infection parameters to control helper-dependent adenoviral vector production
Abstract

Helper-dependent adenoviral vectors (HDVs) are the most promising adenoviral vectors for gene therapy treatments as well as vaccination strategies. However, the lack of a robust and efficient strategy to produce the HDV at high titers constitutes a major obstacle hindering the use of this promising technology at the clinical level. The HDV production requires a double infection of a recombinase-expressing HEK293 cell line with the HDV and a helper virus (HV). To limit the contamination of viral HDV lots by HV, encapsidation of HV is prevented by the recombinase action. A real-time quantitative PCR assay was developed to accurately characterize the contamination of the final product by HV. Infection strategies to enhance the HDV yield and reduce the contamination by HV were investigated. The multiplicity of infection (MOI) was identified as a critical parameter to simultaneously improve the HDV yield and reduce the contamination by HV. HDV-to-HV MOI ratio dictated the HDV yield whereas the HV accumulation was controlled by the MOI of HV. Delaying infection with the HV did not improve the HDV yield.

Keywords
Adenovirus; Helper-dependent adenoviral vector; HEK293 cells; Multiplicity of infection; Time of infection; Real-time qPCR assay; Gene therapy
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Identification of critical infection parameters to control helper-dependent adenoviral vector production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 142, Issue 2, 15 June 2009, Pages 142–150
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us