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Hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film

Paper ID Volume ID Publish Year Pages File Format Full-Text
24591 43528 2009 6 PDF Available
Title
Hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film
Abstract

Nanostructured cerium oxide (NanoCeO2) film deposited onto indium-tin-oxide (ITO) glass substrate by solution casting has been used for immobilization of horseradish peroxidase (HRP) via physiosorption technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), UV–vis and electrochemical techniques have been utilized for characterization of NanoCeO2/ITO electrode and HRP/NanoCeO2/ITO bioelectrode. The HRP/NanoCeO2/ITO electrode exhibits value of the apparent Michaelis–Menten constant (Km) as 2.21 μM, linear regression coefficient as 0.998 and linearity for hydrogen peroxide as 1.0–170 μM obtained using electrochemical response measurements. Besides this, HRP/NanoCeO2/ITO bioelectrode can be used about 20 times and is stable for 5 weeks at 4 °C. The results of photo-response studies carried out on HRP/NanoCeO2/ITO bioelectrode indicate reasonable agreement with those obtained using amperometric technique.

Keywords
Horseradish peroxidase (HRP); NanoCeO2; Biosensor; Hydrogen peroxide
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Hydrogen peroxide sensor based on horseradish peroxidase immobilized nanostructured cerium oxide film
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 142, Issue 2, 15 June 2009, Pages 179–184
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us