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Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Paper ID Volume ID Publish Year Pages File Format Full-Text
24607 43530 2009 7 PDF Available
Title
Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen
Abstract

Hydrogenase-modified electrodes are a promising catalytic surface for the electrolysis of water with an overpotential close to zero. The [FeFe]-hydrogenase CrHydA1 from the photosynthetic green alga Chlamydomonas reinhardtii is the smallest [FeFe]-hydrogenase known and exhibits an extraordinary high hydrogen evolution activity. For the first time, we immobilized CrHydA1 on a gold surface which was modified by different carboxy-terminated self-assembled monolayers. The immobilization was in situ monitored by surface-enhanced infrared spectroscopy. In the presence of the electron mediator methyl viologen the electron transfer from the electrode to the hydrogenase was detected by cyclic voltammetry. The hydrogen evolution potential (−290 mV vs NHE, pH 6.8) of this protein modified electrode is close to the value for bare platinum (−270 mV vs NHE).The surface coverage by CrHydA1 was determined to 2.25 ng mm−2 by surface plasmon resonance, which is consistent with the formation of a protein monolayer. Hydrogen evolution was quantified by gas chromatography and the specific hydrogen evolution activity of surface-bound CrHydA1 was calculated to 1.3 μmol H2 min−1 mg−1 (or 85 mol H2 min−1 mol−1). In conclusion, a viable hydrogen-evolving surface was developed that may be employed in combination with immobilized photosystems to provide a platform for hydrogen production from water and solar energy with enzymes as catalysts.

Keywords
[FeFe]-hydrogenase; Biological hydrogen production; Monolayer; Enzyme-modified electrode; Electron transfer; FTIR; SEIRA
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Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 142, Issue 1, 1 June 2009, Pages 3–9
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us