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Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms

Paper ID Volume ID Publish Year Pages File Format Full-Text
24609 43530 2009 10 PDF Available
Title
Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms
Abstract

Several species of green algae use [FeFe]-hydrogenases to oxidize and/or produce H2 during anoxia. To further define unique aspects of algal hydrogenase activity, the well-studied anaerobic metabolisms of Chlamydomonas reinhardtii were compared with four strains of Chlamydomonas moewusii and a Lobochlamys culleus strain. In vivo and in vitro hydrogenase activity, starch accumulation/degradation, and anaerobic end product secretion were analyzed. The C. moewusii strains showed the most rapid induction of hydrogenase activity, congruent with high rates of starch catabolism, and anoxic metabolite accumulation. Intriguingly, we observed significant differences in morphology and hydrogenase activity in the C. moewusii strains examined, likely the result of long-term adaptation and/or genetic drift during culture maintenance. Of the C. moewusii strains examined, SAG 24.91 showed the highest in vitro hydrogenase activity. However, SAG 24.91 produced little H2 under conditions of sulfur limitation, which is likely a consequence of its inability to utilize exogenous acetate. In L. culleus, hydrogenase activity was minimal unless pulsed light was used to induce significant H2 photoproduction. Overall, our results demonstrate that unique anaerobic acclimation strategies have evolved in distinct green algae, resulting in differential levels of hydrogenase activity and species-specific patterns of NADH reoxidation during anoxia.

Keywords
ACK, acetate kinase; ADH, alcohol dehydrogenase; ADHE, alcohol/aldehyde dehydrogenase; AIB, anaerobic induction buffer; DHAP, dihydroxyacetone phosphate; FDX, ferredoxin; G3P, glycerol 3-phosphate; GAP, glyceraldehyde 3-phosphate; GPD, glycerol 3-phosphat
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Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 142, Issue 1, 1 June 2009, Pages 21–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us