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Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
24729 43533 2008 10 PDF Available
Title
Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis
Abstract

This work investigates to which extent different carbon sources are metabolized and used for lipid biosynthesis in retrovirus producer cells, with the ultimate goal of understanding its importance regarding the stability/productivity of the vectors.For that purpose, isotopically labeled substrates (U-13C glucose, U-13C galactose, U-13C fructose, and U-13C glutamine) were used in combination with 13C nuclear magnetic resonance (NMR) spectroscopy and gas chromatography–mass spectrometry (GC–MS). Our results show that glucose plays the major role in lipid biosynthesis, whereas glutamine, fructose and galactose are not significantly incorporated into lipids. Moreover, a correlation between medium osmolality (imposed by the presence of sorbitol) and virus stability and productivity was verified, apparently due to an enhancement in sugar metabolism.Since low stability and short half-life constitute the major bottleneck in process development for retrovirus and other enveloped viral vectors, this work presents useful knowledge for improved process robustness for these essential gene therapy vectors.

Keywords
Retrovirus vectors production; Carbon sources; Lipid biosynthesis; 13C NMR spectroscopy; Osmolality
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 138, Issues 3–4, 25 November 2008, Pages 57–66
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us